Inhibitory effects of SKF96365 on the activities of K⁺ channels in mouse small intestinal smooth muscle cells
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- TANAHASHI Yasuyuki
- Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603–8555, Japan
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- WANG Ban
- Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603–8555, Japan
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- MURAKAMI Yuri
- Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603–8555, Japan
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- UNNO Toshihiro
- Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
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- MATSUYAMA Hayato
- Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
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- NAGANO Hiroshi
- Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
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- KOMORI Seiichi
- Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
書誌事項
- タイトル別名
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- Inhibitory effects of SKF96365 on the activities of K<sup>+</sup> channels in mouse small intestinal smooth muscle cells
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抄録
In order to investigate the effects of SKF96365 (SKF), which is a non-selective cationic channel blocker, on K+ channel currents, we recorded currents through ATP sensitive K+ (IKATP), voltage-gated K+ (IKv) and Ca2+ activated K+ channels (IBK) in the absence and presence of SKF in single small intestinal myocytes of mice with patch-clamp techniques. SKF (10 µM) reversibly abolished IKATP that was induced by cromakalim (10 µM), which is a selective ATP sensitive K+ channel opener. These inhibitory effects were induced in a concentration-dependent and voltage-independent manner. The 50% inhibitory concentration (IC50) was 0.85 µM, which was obviously lower than that reported for the muscarinic cationic current. In addition, SKF (1 µM ≈ the IC50 value in IKATP suppression) reversibly inhibited the IKv that was induced by repetitive depolarizing pulses from −80 to 20 mV. However, the extent of the inhibitory effects was only ~30%. In contrast, SKF (1 µM) had no significant effects on spontaneous transient IBK and caffeine-induced IBK. These results indicated that SKF inhibited ATP sensitive K+ channels and voltage-gated K+ channels, with the ATP sensitive K+ channels being more sensitive than the voltage-gated K+ channels. These inhibitory effects on K+ channels should be considered when SKF is used as a cationic channel blocker.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 78 (2), 203-211, 2016
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206429329152
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- NII論文ID
- 130005131348
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 027141229
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- PubMed
- 26498720
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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- 使用不可