Diversity of Fimbrillin among <i>Porphyromonas gulae</i> Clinical Isolates from Japanese Dogs

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  • NOMURA Ryota
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565–0871, Japan
  • SHIRAI Mitsuyuki
    Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
  • KATO Yukio
    Department of Veterinary Public Health II, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
  • MURAKAMI Masaru
    Department of Molecular Biology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
  • NAKANO Kazuhiko
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565–0871, Japan
  • HIRAI Norihiko
    Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
  • MIZUSAWA Tetsuya
    Department of Veterinary Public Health II, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan
  • NAKA Shuhei
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565–0871, Japan
  • YAMASAKI Yoshie
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565–0871, Japan Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700–8556, Japan
  • MATSUMOTO-NAKANO Michiyo
    Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700–8556, Japan
  • OOSHIMA Takashi
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka 565–0871, Japan
  • ASAI Fumitoshi
    Department of Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252–5201, Japan

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  • Diversity of Fimbrillin among Porphyromonas gulae Clinical Isolates from Japanese Dogs

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Porphyromonas gulae, a gram-negative black-pigmented anaerobe, is a pathogen for periodontitis in dogs. An approximately 41-kDa fimbrial subunit protein (FimA) encoded by fimA is regarded as associated with periodontitis. In the present study, the fimA genes of 17 P. gulae strains were sequenced, and classified into two major types. The generation of phylogenetic trees based on the deduced amino acid sequence of FimA of P. gulae strains along with sequences from several strains of Porphyromonas gingivalis, a major cause of human periodontitis, revealed that the two types of FimA (types A and B) of P. gulae were similar to type I FimA and types II and III FimA of P. gingivalis, respectively. A PCR system for classification was established based on differences in the nucleotide sequences of the fimA genes. Analysis of 115 P. gulae-positive oral swab specimens from dogs revealed that 42.6%, 22.6%, and 26.1% of them contained type A, type B, and both type A and B fimA genes, respectively. Experiments with a mouse abscess model demonstrated that the strains with type B fimA caused significantly greater systemic inflammation than those with type A. These results suggest that the FimA proteins of P. gulae are diverse with two major types and that strains with type B fimA could be more virulent.

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