Rapid Identification of Hemoplasma Species by Palindromic Nucleotide Substitutions at the GAAA Tetraloop Helix in the Specificity Domain of Ribonuclease P RNA

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  • SASAOKA Fumina
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
  • SUZUKI Jin
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University
  • WATANABE Yusaku
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University Department of Applied Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
  • FUJIHARA Masatoshi
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University Department of Applied Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
  • HARASAWA Ryô
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University Department of Applied Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University

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  • Bacteriology : Rapid Identification of Hemoplasma Species by Palindromic Nucleotide Substitutions at the GAAA Tetraloop Helix in the Specificity Domain of Ribonuclease P RNA

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We examined secondary structures of the ribonuclease P RNA sequences obtained from DNA databases, and identified a determinative prototype of the P12 helix peculiar to each species of hemoplasmas. This key structure will provide a rapid means for species identification of these uncultivable pathogens without making a phylogenetic tree based on alignments of nucleotide sequences. This procedure based on palindromic nucleotide substitutions at the stem portion of the P12 helix provide clear information such as the level of heterogeneity within a species, the relatedness between species, or facilitating the characterization and clustering of specific strains. In conclusion, the PNS analysis is based on the evaluation of only the strategic and highly conserved genomic region in the specificity domain of RNase P RNA.<br>

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