Pharmacology: Influences of organic solvents on CYPMPO-electron spin resonance spectra in in vitro radical generating systems
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- MUKOHDA Masashi
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University
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- UENO Shunji
- Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University
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- KAMIBAYASHI Masato
- Kyoto Pharmaceutical University
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- OKADA Muneyoshi
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University
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- YAMAWAKI Hideyuki
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University
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- HARA Yukio
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University
書誌事項
- タイトル別名
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- Influences of Organic Solvents on CYPMPO-Electron Spin Resonance Spectra in In Vitro Radical Generating Systems
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抄録
Estimation of radical scavenging capacity of lipophilic antioxidants by electron spin resonance (ESR) in vitro is a challenging issue due to their poor solubility in aqueous radical generating and measuring systems. Water-miscible organic solvents are used for this purpose. A novel radical trapping agent, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO), that has practical advantages over well-known trapping agents was synthesized. However, no available data for the influence of solvents in an ESR system that uses CYPMPO has been presented. The influences of six water-miscible organic solvents, acetonitrile (AcN), acetone, dimethyl sulfoxide (DMSO), ethanol, polyethylene glycol (PEG), and dimethoxyethane (DME), on ESR responses to Fenton Fe2+/H 2O2 OH· and hypoxanthine/xanthine oxidase superoxide generation systems in vitro were studied. Reduction of the ESR signal to CYPMPO-OH· adducts by 55.86 ± 5.95 and 83.17 ± 2.50% compared with the control was observed in the presence of AcN and acetone, respectively, at a final concentration of 5% (v/v). AcN of less than 1% had minimal effects. DMSO, ethanol, PEG and DME at 5% (v/v) strongly inhibited the ESR signals and/or caused derangement in the signal patterns. The six water-miscible solvents at 5% (v/v) had no influence on the ESR spectra of CYPMPO-superoxide adducts. From these results, AcN, at less than 1% (v/v), is a useful water-miscible organic solvent for assessing radical scavenging capacities of lipophilic compounds in the CYPMPO-Fenton Fe2+/H2O2 OH· reaction system in an ESR assay. Any of the solvents used in the present study can be used in a hypoxanthine/xanthine oxidase superoxide generation system.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 72 (12), 1547-1550, 2010
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206429541248
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- NII論文ID
- 130000307683
- 80021550419
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BC3MXhtlOgurc%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 10954213
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- PubMed
- 20661003
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可