Parasitology : Seroprevalences of Toxoplasma gondii and Neospora caninum in Pet Rabbits in Japan
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- SALMAN Doaa
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080–8555, Japan Department of Animal Medicine, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
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- OOHASHI Eiji
- Akashiya Animal Hospital, Makubetsu, Hokkaido 089–0535, Japan
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- MOHAMED Adel Elsayed Ahmed
- Department of Animal Medicine, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
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- ABD EL-MOTTELIB Abd El-Raheem
- Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
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- OKADA Tadashi
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080–8555, Japan
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- IGARASHI Makoto
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080–8555, Japan
書誌事項
- タイトル別名
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- Seroprevalences of <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in Pet Rabbits in Japan
- Seroprevalences of <italic>Toxoplasma gondii</italic> and <italic>Neospora caninum</italic> in Pet Rabbits in Japan
この論文をさがす
抄録
The potential contamination of Toxoplasma gondii and Neospora caninum oocysts in the human environment is a concern from the public health viewpoint. However, estimation of their seroprevalences in humans cannot be performed in a manner that distinguishes between oocysts and tissue cysts as a source of infection. Rabbits are considered popular pet animals in Japan that can acquire natural infections by the aforementioned parasites only through the ingestion of oocysts. Therefore, this study was conducted to estimate the seroprevalences of T. gondii and N. caninum in pet rabbits in Japan as an indicator of the possible oocyst contamination in the environment surrounding human beings. Serum samples of 337 rabbits were examined by different serological methods. Enzyme-linked immunosorbent assays were performed to measure the titer of IgG and IgM antibodies. Samples revealed to be seropositive by ELISA were further analyzed by a latex agglutination test, Western blotting and an indirect immunofluorescence assay. The rates of seropositivity for T. gondii were 0.89% (3/337) and 0.29% (1/337) in IgG and IgM ELISA, respectively. SAG1 and SAG2 were detected as major antigens by the positive rabbit sera in Western blotting associated with strong staining observed by IFA in T. gondii tachyzoites. Regarding N. caninum, none of the serum samples showed a specific reaction in both Western blotting and the IFA. The results of this study indicate low seroprevalences of toxoplasmosis and neosporosis in pet rabbits in Japan, suggesting low oocyst contamination in the human environment.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 76 (6), 855-862, 2014
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206429750272
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- NII論文ID
- 130003391296
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC2cvpt1ehtQ%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 025618111
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- PubMed
- 24584081
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可