Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens
-
- SUZUKI Kazuya
- Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
-
- OGUMA Keisuke
- Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
-
- SENTSUI Hiroshi
- Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
Search this article
Description
<p>We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum.</p>
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 79 (1), 141-143, 2017
JAPANESE SOCIETY OF VETERINARY SCIENCE