DNA Damage and Decrease of Cellular Oxidase Activity in Piglet Sertoli Cells Exposed to Arsanilic Acid
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- ZHANG Ming
- College of Veterinary Medicine, Hunan Agricultural University Jiangxi Biotech Vocational College
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- HE Zuping
- Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center
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- YUAN Hui
- College of Veterinary Medicine, Hunan Agricultural University
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- ZHU Li
- College of Veterinary Medicine, Hunan Agricultural University
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- GUO Chengzhi
- College of Veterinary Medicine, Hunan Agricultural University
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- YIN Lu
- College of Veterinary Medicine, Hunan Agricultural University
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- WU Jing
- College of Veterinary Medicine, Hunan Agricultural University
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- DENG Sijun
- College of Veterinary Medicine, Hunan Agricultural University
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- YUAN Liyun
- College of Veterinary Medicine, Hunan Agricultural University
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- WEN Lixin
- College of Veterinary Medicine, Hunan Agricultural University
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説明
The study was designed to explore the toxic effects of arsanilic acid on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion followed by differential plating. Piglet Sertoli cells were cultured and classified into the following five groups: group A, the control without arsanilic acid treatment; group B, cultured with 5 μM arsanilic acid; group C, cultured with 50 μM arsanilic acid; group D, cultured with 0.5 mM arsanilic acid; and group E, cultured with 5 mM arsanilic acid. We found that Sertoli cell growth was inhibited by arsanilic acid at 0.5 mM compared with the control, group A. The oxidase activity of Sertoli cells was decreased by arsanilic acid at 0.5 mM as evidenced by the observations that arsanilic acid increased MDA content but decreased the SOD and GSH-Px activities of Sertoli cells. Moreover, 50 μM of arsanilic acid was observed to cause DNA damage in Sertoli cells. The results of our study suggest that exposure of Sertoli cells to arsanilic acid leads to induction of oxidative stress and inhibition of cell growth at a high concentration, while arsanilic acid causes DNA damage in Sertoli cells at a low concentration.<br>
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 73 (2), 199-203, 2011
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206430280832
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- NII論文ID
- 130000444135
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- ISSN
- 13477439
- 09167250
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可