Anatomy: Nitric oxide production and its contribution to hepatocyte proliferation in normal juvenile rats
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- INUKAI Naoto
- Graduate School of Veterinary Medicine, Azabu University
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- UCHIDA Mayuko
- Graduate School of Veterinary Medicine, Azabu University
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- MIYAZAKI Yoko
- Graduate School of Veterinary Medicine, Azabu University
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- SUZUKI Takehito
- Graduate School of Veterinary Medicine, Azabu University
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- YOSHIKAWA Hiroshi
- Research Institute of Biosciences, Azabu University
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- TANAKA Kazuaki
- Graduate School of Veterinary Medicine, Azabu University
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- MORITA Hidetoshi
- Graduate School of Veterinary Medicine, Azabu University Research Institute of Biosciences, Azabu University
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- TAKIZAWA Tatsuya
- Graduate School of Veterinary Medicine, Azabu University Research Institute of Biosciences, Azabu University
書誌事項
- タイトル別名
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- Nitric Oxide Production and its Contribution to Hepatocyte Proliferation in Normal Juvenile Rats
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抄録
Nitric oxide (NO) has been reported as a key mediator in enhancing hepatocyte proliferation during liver regeneration. Juvenile hepatocytes have a strong ability to proliferate while still in their undifferentiated state but the mechanism of NO production and its contribution to hepatocyte proliferation are not yet fully understood. The present study was designed to investigate NO production in the normal liver and its contribution to hepatocyte proliferation in juvenile rats. Endogenous NO production was evaluated quantitatively using a spin trap followed by electron paramagnetic resonance spectroscopy with the Fe-N, N-diethyldithiocarbamate complex as an NO-trapping reagent in the rat liver. NO production in the liver significantly peaked at 3 weeks after birth, but NO synthase (NOS) 3 expression did not change between 2 to 5 weeks after birth, while NOS 1 and NOS 2 mRNA were not detected. Hepatocyte proliferation, measured by the incorporation of 5-bromo-2'-deoxyuridine into the DNA, was found to decline significantly when endogenous NO production was inhibited by the administration of the NOS inhibitor NG-nitro- L-arginine methyl ester. These findings indicate that endogenous NO production peaked at 3 weeks after birth and hepatocyte proliferation declined significantly when NO production was inhibited. Thus, this study provides a novel insight into the contribution of NO to hepatic growth and liver maturation in juveniles.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 72 (7), 861-867, 2010
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206430356608
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- NII論文ID
- 130000264281
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 10782945
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可