Biochemistry: Parallel reductions in stomatin and Na,K-ATPase through the exosomal pathway during reticulocyte maturation in dogs: stomatin as a genotypic and phenotypic marker of high K[+] and low K[+] red cells
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- KOMATSU Tomohiko
- Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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- SATO Kota
- Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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- OTSUKA Yayoi
- Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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- ARASHIKI Nobuto
- Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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- TANAKA Kohei
- Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- TAMAHARA Satoshi
- Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- ONO Ken-ichiro
- Laboratory of Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- INABA Mutsumi
- Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University
書誌事項
- タイトル別名
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- Parallel Reductions in Stomatin and Na,K-ATPase through the Exosomal Pathway during Reticulocyte Maturation in Dogs: Stomatin as a Genotypic and Phenotypic Marker of High K+ and Low K+ Red Cells
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Dogs can be divided into two genetic groups (a minor HK phenotype and a major LK phenotype) based on erythrocyte monovalent cation concentrations, which are controlled by the putative hk and lk allelic genes. HK dogs retain Na,K-ATPase in their erythrocytes due to the high activity of the enzyme in their precursor cells, whereas total loss of reticulocyte Na,K-ATPase occurs in LK dogs. Here, we report that the levels of the lipid raft-associated membrane protein stomatin decrease in parallel with those of Na,K-ATPase during reticulocyte maturation due to its extrusion in exosomes. The stomatin content of HK reticulocytes is higher than that of LK reticulocytes, and remains in the erythrocytes at levels compatible with that in human erythrocytes. However, it is almost absent from LK erythrocytes with the lk/lk genotype; similar to the deficiency seen in human red cells with overhydrated stomatocytosis. LK erythrocytes from hk/lk genotype dogs show reduced, but not negligible, levels of stomatin. These results indicate that the erythrocyte stomatin level is a suitable genotypic marker for the HK/LK red cell phenotype, and suggests a functional association between stomatin and Na,K-ATPase. The absence of morphological abnormalities in the erythrocytes of stomatin-deficient LK dogs also confirms that stomatin deficiency and stomatocytic shape change are independent from each other.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 72 (7), 893-901, 2010
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206430371328
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- NII論文ID
- 130000264273
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 10783007
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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