Pathology: Immunohistochemical detection of disease-associated prion protein in the intestine of cattle naturally affected with bovine spongiform encephalopathy by using an alkaline-based chemical antigen retrieval method
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- OKADA Hiroyuki
- Prion Disease Research Center, National Institute of Animal Health
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- IWAMARU Yoshihumi
- Prion Disease Research Center, National Institute of Animal Health
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- IMAMURA Morikazu
- Prion Disease Research Center, National Institute of Animal Health
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- MASUJIN Kentaro
- Prion Disease Research Center, National Institute of Animal Health
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- YOKOYAMA Takashi
- Prion Disease Research Center, National Institute of Animal Health
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- MOHRI Shirou
- Prion Disease Research Center, National Institute of Animal Health
書誌事項
- タイトル別名
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- Immunohistochemical Detection of Disease-Associated Prion Protein in the Intestine of Cattle Naturally Affected with Bovine Spongiform Encephalopathy by Using an Alkaline-Based Chemical Antigen Retrieval Method
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説明
An alkaline-based chemical antigen retrieval pretreatment step was used to enhance immunolabeling of disease-associated prion protein (PrPSc) in formalin-fixed and paraffin-embedded tissue sections from cattle naturally affected with bovine spongiform encephalopathy (BSE). The modified chemical method used in this study amplified the PrPSc signal by unmasking PrPSc compared with the normal cellular prion protein. In addition, this method reduced nonspecific background immunolabeling that resulted from the destruction of the residual normal cellular form of prion protein, and reduced the treatment time compared with the usual autoclave pretreatment step. Immunolabeled PrPSc was thereby clearly detected in the myenteric plexus of the ileum in naturally occurring BSE cattle.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 72 (11), 1423-1429, 2010
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206430410496
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- NII論文ID
- 130000307667
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BC3MXkt1Gh
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 10918874
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- PubMed
- 20606369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可