Identification of Novel Immunogenic Proteins in <i>Mycoplasma capricolum</i> subsp. <i>Capripneumoniae</i> Strain M1601
-
- ZHAO Ping
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- HE Ying
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- CHU Yue-feng
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- GAO Peng-cheng
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- ZHANG Xuan
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- ZHANG Nian-zhang
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- ZHAO Hai-yan
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- ZHANG Ke-shan
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
-
- LU Zhong-xin
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Bibliographic Information
- Other Title
-
- Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp. Capripneumoniae Strain M1601
Search this article
Abstract
The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29–97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 74 (9), 1109-1115, 2012
JAPANESE SOCIETY OF VETERINARY SCIENCE
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206430562688
-
- NII Article ID
- 130001879755
-
- NII Book ID
- AA10796138
-
- COI
- 1:STN:280:DC%2BC38npsFarsg%3D%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL BIB ID
- 024012112
-
- PubMed
- 22673397
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed