Canine Amino Acid Transport System X<sub>c</sub><sup>–</sup>: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

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  • MARUO Takuya
    Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
  • KANEMAKI Nobuyuki
    Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
  • ONDA Ken
    Laboratory of Internal Medicine 3, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
  • SATO Reiichiro
    Laboratory of Gynecology and Obstetrics, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
  • ICHIHARA Nobuteru
    Laboratory of Anatomy 1, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
  • OCHIAI Hideharu
    Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan

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  • Canine Amino Acid Transport System Xc⁻ : cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

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Abstract

The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.

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