Improvement of Preimplantation Development of <i>In Vitro-</i>Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

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  • SAKAGAMI Nobutada
    Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
  • NISHINO Osamu
    Nara Prefectural Livestock Technology Center, Nara 633–1302, Japan
  • ADACHI Satoshi
    Ooita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878–0201, Japan
  • UMEKI Hidenobu
    Ooita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878–0201, Japan
  • UCHIYAMA Hiroko
    Miyazaki Prefectural Livestock Research Institute, Miyazaki 889–4411, Japan
  • ICHIKAWA Kyoko
    Kochi Prefectural Livestock Research Institute, Kochi 789–1233, Japan
  • TAKESHITA Kazuhisa
    Yamaguchi Prefectural Livestock Experiment Station, Yamaguchi 759–2221, Japan
  • KANEKO Etsushi
    Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
  • AKIYAMA Kiyoshi
    Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
  • KOBAYASHI Shuji
    National Livestock Breeding Center, Fukushima 961–8511, Japan
  • TAMADA Hiromichi
    Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 598–8531, Japan

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  • Theriogenology : Improvement of Preimplantation Development of In Vitro-Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

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Description

The influences of glucose supplementation on early development of bovine embryos in BSA-free synthetic oviduct fluid were examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose either at 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in the group supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controls without glucose supplementation. The results suggest that appropriate amounts of glucose supplemented to the medium at the specific stage of embryo culture may be useful for the production of bovine blastocysts.

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