Identification of Matrix Metalloproteinase-2 and -9 Activities within Intestinal Mucosa of Clinically Healthy Beagle Dogs

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  • HANIFEH Mohsen
    Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), 00014 Helsinki, Finland Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Postal Code 5166616471, Tabriz, Iran
  • RAJAMÄKI Minna M
    Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), 00014 Helsinki, Finland
  • MÄKITALO Laura
    Children’s Hospital, Helsinki University Central Hospital and University of Helsinki, P.O. Box 63 (Haartmaninkatu 8), 00014 Helsinki, Finland
  • SYRJÄ Pernilla
    Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66 (Agnes Sjöbergin katu 2), 00014 Helsinki, Finland
  • SANKARI Satu
    Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), 00014 Helsinki, Finland
  • KILPINEN Susanne
    Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), 00014 Helsinki, Finland
  • SPILLMANN Thomas
    Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), 00014 Helsinki, Finland

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  • Internal Medicine : Identification of Matrix Metalloproteinase-2 and -9 Activities within Intestinal Mucosa of Clinically Healthy Beagle Dogs

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Abstract

Matrix metalloproteinases (MMPs) 2 and 9 are zinc-dependent endopeptidases that contribute to the control of breakdown and reconstitution of extracellular matrix under both normal and pathological conditions. The main objective of this study was to identify the presence of MMP-2 and -9 in the mucosa of the small and large intestines of clinically healthy beagle dogs using gelatin zymography technique. Intestinal mucosa samples from four different parts of the intestine (duodenum, jejunum, ileum and colon) were taken from 12 healthy laboratory beagle dogs and examined histologically. Based on WSAVA histology standards, recorded findings of all samples were considered insignificant. Pro-MMP-2 and -9 activities were found in 17/48 (35%) and 25/48 (52%) of the samples, respectively. Among four different parts of the intestine of 12 dogs, the ileum had the highest positivity rates of 7/12 (58.3%) and 8/12 (66.7%) for pro-MMP-2 and -9 activities, respectively. However, statistical analysis showed no significant difference of pro-MMP-2 and -9 activities between the separate parts of the intestine (P>0.05). None of the intestinal samples showed gelatinolytic activity corresponding to the control bands of active MMP-2 and MMP-9. This study showed that pro-MMP-2 and -9 could be detected in the intestinal mucosa of healthy dogs using zymography, which seems to be a useful tool to evaluate the role of MMP-2 and -9 in the pathogenesis of canine chronic enteropathies, including inflammatory bowel diseases.

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