Ultrastructural Study on Colocalization of Glucagon-Like Peptide (GLP)-1 with GLP-2 in Chicken Intestinal L-Cells
-
- NISHIMURA Kei
- Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
-
- HIRAMATSU Kohzy
- Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
-
- MONIR Mohammad M.
- Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
-
- TAKEMOTO Chihiro
- Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
-
- WATANABE Takafumi
- Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
Search this article
Description
Colocalization of glucagon-like peptide (GLP)-1 with GLP-2 in L-cells was investigated in the chicken ileum by using double immunofluorescent and immunocytochemical techniques. Ultrastructural features of L-cells were also clarified in this study. L-cells showing immunoreactivity for both GLP-1 and GLP-2 were distributed in the whole ileum. They showed comma-like or flask-like shape and were located in epithelium of crypts and lower part of intestinal villi. L-cells showing GLP-1-immunoreactivity only were found in epithelium of lower and middle parts of intestinal villi. Transmission electron microscopy indicated that L-cells identified by colloidal gold-labeled immunocytochemistry were covered apically with microvilli, open-type and contained many secretory granules in their perikarya. These secretory granules without halo were round to oval in shape and showed moderate electron density. The longest and shortest diameters of secretory granules were 355 ± 62 nm (mean ± SD) and 287 ± 48 nm, respectively. Double labeling immunocytochemistry using two different sizes of particles (6 and 12 nm in diameter) of colloidal gold revealed that GLP-1 colocalized with GLP-2 in the same secretory granules. This study advances new morphological data about the endocrine system of the chicken small intestine.
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 75 (10), 1335-1339, 2013
JAPANESE SOCIETY OF VETERINARY SCIENCE
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206431040384
-
- NII Article ID
- 130003362229
-
- NII Book ID
- AA10796138
-
- COI
- 1:STN:280:DC%2BC3sjhtlehsg%3D%3D
-
- ISSN
- 13477439
- 09167250
-
- HANDLE
- 10091/17855
-
- NDL BIB ID
- 024963989
-
- PubMed
- 23759686
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed