Small Interfering RNA (siRNA) against the Survivin Gene Increases Apoptosis in a Canine Melanoma Cell Line
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- MORIYAMA Miyuki
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- KANO Rui
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- MARUYAMA Haruhiko
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- HASEGAWA Atsuhiko
- Teikyo University Institute of Medical Mycology
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- KAMATA Hiroshi
- Department of Pathobiology, Nihon University School of Veterinary Medicine
Bibliographic Information
- Other Title
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- Clinical pathology: Small interfering RNA (siRNA) against the survivin gene increases apoptosis in a canine melanoma cell line
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Abstract
The effect of down-regulation of survivin expression by small interfering RNA (siRNA) against the canine survivin gene on apoptosis was investigated by transfecting MCM-N1 (a canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine survivin gene induced an increase in the rate of apoptotic cells and a decrease in the number of viable cells. We also measured sequence-specific down-regulation of survivin expression by reverse transcription-PCR and western blot analysis. The siRNA directed against survivin reduced both mRNA and protein expression in MCM-N1 cells. These findings suggest the importance of survivin in canine melanoma tumors for inducing apoptosis, and reinforce the possibility of using survivin as a putative therapeutic target in canine malignant melanoma tumor.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 72 (12), 1643-1646, 2010
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206431063680
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- NII Article ID
- 130000307688
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 10954435
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed