Evaluation of Immunochromatographic Assay for Serodiagnosis of Brucella canis
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- KIM Jong Wan
- National Veterinary Research and Quarantine Service
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- LEE Young Ju
- College of Veterinary Medicine, Kyungpook National University
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- HAN Moo Yeol
- College of Veterinary Medicine, Kyungpook National University
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- BAE Dong Hwa
- College of Veterinary Medicine, Kyungpook National University
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- JUNG Suk Chan
- National Veterinary Research and Quarantine Service
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- OH Jin Sik
- Animal Genetics, Inc.
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- HA Gun Woo
- Animal Genetics, Inc.
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- CHO Byung Ki
- Animal Genetics, Inc.
Bibliographic Information
- Other Title
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- Public health: Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis
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Abstract
Canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. Diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. The objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ICA) for serodiagnosis of dogs suspected of having brucellosis, and results were compared with those obtained for hemoculture (HC) and the rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT). The all experimentally infected dogs were positive in ICA, HC and 2-ME RSAT from 5 weeks, 7 weeks, and 3 weeks after infection, respectively. Also, among dogs selected from 10 different breed kennels occurred brucellosis, 24.8%, 39.5% and 39.1% of dogs tested were detected as positive with HC, 2-ME RSAT and ICA, respectively. The kappa value between 2-ME RSAT and ICA was 0.89. The results of this study showed that sensitivity and specificity of the ICA are comparable with those obtained using conventional serological and bacteriological test for brucellosis. In conclusion, the ICA kit provides a handy and accurate tool for the rapid serodiagnosis of canine brucellosis.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 69 (11), 1103-1107, 2007
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206431390976
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- NII Article ID
- 110006452645
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 9281938
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed