Strain establishment and histogenesis of transplantable human malignant fibrous histiocytoma of the maxilla into nude mouse.

DOI
  • OHSE Shusaku
    Department of Oral Surgery, School of Medicine, Mie University

Bibliographic Information

Other Title
  • ヌードマウス可移植性ヒト上顎由来悪性線維性組織球腫累代株の樹立とその組織起源

Abstract

Serial transplantable strains of nude mouse derived human malignant fibrous histiocytoma (MFH) originating in maxilla were established and the histogenesis was studied.<BR>The tumor obtained from the recurrence of a MFH originated in the right maxilla of a 60-year-old woman. It was transplanted beneath the scapular skin in nude mice and was transferred for over 4 and a half years for 37 consecutive passages (the take rate was 220/237, 92.8%) to establish a nude mouse-transplantable MFH. In the tumors of the 15th and 34th passages, the chromosomes were analyzed and the human origin was confirmed. By light microscopic observation, polygonal histiocyte-like cells characterized by polymorphism and atypism were observed. Acid phosphatase, naphtol AS-D acetate esterase were positive by enzymatic histochemistry testing, while lysozyme, α 1-antitrypsin, and ai-antichymotrypsin were positive immunohistochemically. On electron microscopic observation, the transferred tumors were mainly composed of histiocyte-like cells. The cell membranes with filopodia, rough endoplasmic reticulum, and many lysosomes having a moderate electron density were observed in the cytoplasm, suggesting the ability of phagocytosis. Undifferentiated cells were not observed.<BR>From these facts it appeared that the MFH would be originated from histiocytes.

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Details 詳細情報について

  • CRID
    1390001206434648320
  • NII Article ID
    130004335168
  • DOI
    10.11277/stomatology1952.38.425
  • ISSN
    21850461
    00290297
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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