書誌事項
- タイトル別名
-
- Radioimmunoassay of Vasopressin in Unextracted Random Urine; Clinical Application for Screening of Central Diabetes Insipidus
- Radioimmunoassay of vasopressin in unextracted random urine; Clinical application for screening of central diabetes inspidus
この論文をさがす
説明
The concentration of unextracted urinary arginine vasopressin (UAVP) was directly measured by high-sensitive radioimmunoassay (AVP-RIA Kit, Mitsubishi Petrochemical Co., Ltd.). Urine was diluted to eliminate interference of nonspecific substance without prior extraction. When urine aliquots were diluted in 4 to 32 fold in assay buffer, the relationship between UAVP concentration and dilution ratio corresponded exactly in a linear regression line. The elution pattern on Sephadex G-25 of UAVP immunoreactivity was identical with that of synthesized AVP. The AVP concentration in unextracted urine was not significantly different from that of extracted urine by Sep-Pak C18 column (Water Associates, Milford MA). The mean recovery of added AVP to urine specimens was 101.1±9.8% (mean±SD). The immunoreactivity of UAVP was not modified by either albuminuria (50 and 100mg/dl) or glycosuria (1000mg/dl). Mean coefficients of variance between-assay and within-assay were 8.3% and 6.6% respectively.<BR>In normal subjects (n=28), significant correlation was observed between UAVP con-centration and simultaneously measured plasma AVP (r=0.701, p<0.001). Moreover, AVP concentration in random urine was significantly correlated with AVP excretion in 24hr-urine (r=0.703, p<0.05, n=9), and this suggested that random UAVP concentration may indicate daily UAVP secretion.<BR>In normal subjects, AVP concentration in random urine was widely scattered from 9.2 to 470.6pg/mg Cr (89.5±76.4pg/mg Cr, n=211). In patients with diabetes insipidus (DI), UAVP concentration (1.6 to 13.0pg/mg Cr, 6.94±2.77pg/mg Cr, n=25) was significantly lower (p<0.001) than that of normal subjects. UAVP concentration in a patient with primary polydipsia (43.2 pg/mg Cr) was not similar to that of ID but to that of normal subjects. UAVP concentration in 2 patients with SIADH was not more than that of normal subjects, indicating that random UAVP concentration is not suitable for detecting inappropriate AVP secretion.<BR>In this study, it is suggested that patients of random UAVP concentration below 13.0 pg/mg Cr should be recommended other intensive examination to diagnose DI, even though 2 normal subjects (0.9%) were incorrectly estimated as DI.<BR>In conclusion, radioimmunoassay of AVP in unextracted random urine is easy to sample and assay, and useful in screening polyuric patients.
収録刊行物
-
- Folia Endocrinologica Japonica
-
Folia Endocrinologica Japonica 65 (5), 537-548, 1989
The Japan Endocrine Society
