臨床用血色素計の製作並びに檢定の基礎

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  • Basic Experiments on the Manufacture and Examination of clinical Hemometers
  • リンショウヨウ ケッシキソケイ ノ セイサク ナラビニ ケンテイ ノ キソ

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1. Basic experiments on the manufacture and examination of clinical hemometers are here described.<br>2. Hemoglobin was determined by analysis for total iron, the concentration being calculated from the iron content of 0.34 per cent. In parallel with the iron method cyanmethemoglobin of the same blood samples was determined in the photoelectric spectrop-hotometer at 540mμ with a wave width of 0.5mμ and adsorption cell of 1.0cm in thickness. Then the constant with which one can calculate the hemoglobin concentration from the optical dencity of cyanmethemoglobin was determined as 0.146.<br>3. The constant for the optical dencity of acid hematin in the spectrophotometer at 520mμ with a wave width of 0.5mμ and absorption cell of 1.0cm in thickness was calculated to be 0.143 by reference to the mean values of hemoglobin concentration obtained from the cyanmethemoglobin method. The hemoglobin concentration of standard blood was determined to be 16g/dl=100 per cent, from which standard solution of acid hematin was prepared.<br>4. The colorimetric properties of the acid hematin solution were studied, and optimal conditions for optical colorimetry were demonstrated as follows: Brightness=18° (or 45 per cent), Saturation=56 per cent, Dominant wave length=581.5mμ, Hue; x'. 0.435, y.' 0.400.<br>5. Salicylic acid added to the acid hematin solution to the extent of 0.01 per cent did not change the color of the solution, and prevented the decoloration of the solution for over one month.<br>6. The light of 1000-5000 Lux from a north window was suitable for optical colori-matry of the acid hematin solution.

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