Computer vision system for enumerating the eggs of a parasitic wasp, Apanteles glomeratus L.

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  • コンピュータ画像処理によるアオムシコマユバチの蔵卵数の自動計測
  • コンピュータ ガゾウ ショリ ニ ヨル アオムシコマユバチ ノ ゾウランスウ

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Abstract

A computer vision system driven by a robot was developed to enumerate and measure the size and number of eggs of a parasitic wasp, Apanteles glomeratus L., which were scattered on a slide. The procedure is as follows. 1) The eggs were stained with methylene blue, the area of interest was circled with a wide yellow boundary line to reduce unnecessary scanning and the slide was overload on a transparent film dotted with a regular array of red markers to define the measurement frame in each scene. 2) The eggs, the boundary area and the markers could be distinguished from each other by their color. Then, the number and the size of the eggs were measured, even considering the overlap of two or more eggs. 3) The stage was driven by the robot to scan all the frames within the boundary area and the results of the measurements were accumulated in the supervising microcomputer. The results of the experiment showed that the reproducibility and accuracy of this system were as good as those achieved by human observation. The ability of the system to scan, enumerate and measure all the particles scattered in a large area can be easily applied to various objectives in different disciplines.

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