Establishment and Maintenance of High Resistance to Bacillus thuringiensis Formulations in Diamondback Moth, Plutella xylostella(Lepidoptera: Yponomeutidae).

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  • Ｂａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓ製剤に対する高度抵抗性コナガ系統の育成と維持法
  • Bacillus thuringiensis セイザイ ニ タイスル コウド テイコウセイ コナガ ケイトウ ノ イクセイ ト イジホウ

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Abstract

Establishment and maintenance of high resistance to Bacillus thuringiensis formulations (BT) in the diamondback moth, Plutella xylostella were investigated using the diet incorporation method. Before beginning the selection, a field BT-resistant colony (ROO) collected from watercress in Kishiwada City (Osaka) was crossed with a susceptible colony (KUS) adapted to artificial diet, because the ROO colony could not be reared on the diet. Progenies from the reciprocal crosses of the ROO and KUS colonies were used for laboratory selection. Two BT formulations, Toarow®-CT and one with Cry1Ac toxin were used independently for selection by incorporation into the diet. An extremely high level of resistance to each BT formulation was obtained within 10 generations by increasing the selection pressure. The resistance ratios of resistant colonies selected by Toarow®-CT and Cry1Ac were >30, 000 and >45, 000, respectively, when compared to the KUS colony. When these two BT-resistant colonies were maintained without selection pressure, the resistance level was maintained for 20 generations, and then decreased gradually.

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