家蚕核多角体病ウイルス温度感受性変異株の分離と性状
書誌事項
- タイトル別名
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- Isolation of Temperature-Sensitive Mutants of Bombyx mori Nuclear Polyhedrosis Virus.
- カサン カクタカクタイビョウ ウイルス オンド カンジュセイ ヘンイカブ ノ
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抄録
Six temperature-sensitive mutants (designated TS1-TS6) of Bombyx mori nuclear polyhedrosis virus (BmNPV) were isolated and three were characterized. In BmN4 cells infected with each ts mutant, occlusion bodies were produced normally, but extracellular virus was produced in reduced amounts at 33°C. The ts mutant adsorption properties of TS3 were similar to the wild type but TS1 and TS2 showed lower levels. SDS-PAGE analysis showed that some polypeptides synthesized in the cells infected with TS2 and TS3 were different from those of the wild type virus. In these ts mutants, such alterations did not depend on the incubation temperature of cultured cells. Restriction endonuclease analysis of viral DNA of ts mutants suggested that most of the mutations occurred in the regions where homologous regions of BmNPV were localized, and the positions of these regions on the genome were similar to those of AcMNPV ts mutants.
収録刊行物
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- 日本応用動物昆虫学会誌
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日本応用動物昆虫学会誌 39 (1), 33-37, 1995
日本応用動物昆虫学会
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詳細情報 詳細情報について
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- CRID
- 1390001206449230592
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- NII論文ID
- 110001113534
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- NII書誌ID
- AN00186121
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- ISSN
- 13476068
- 00214914
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- NDL書誌ID
- 3603472
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可