抗生物質ダニ剤Ｍｉｌｂｅｍｙｃｉｎｓに関する研究　ＩＩ　　Ｍｉｌｂｅｍｙｃｉｎｓで室内とうたしたナミハダニの性質

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タイトル別名
  • Acaricidal Studies on Milbemycins, a New Family of Macrolide Antibiotics
  • コウセイ ブッシツ ダニザイ Milbemycins ニカンスルケンキュウ 2
  • 2. Milbemycinsで室内淘汰したナミハダニの性質

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A milbemycins-resistant strain (MR-strain) of the two-spotted spider mite, which was 100-170-fold as resistant as the parent susceptible strain (Sapporo S-strain) in the adult stage, and 10-fold in one-day old egg was developed through 24 selections with LC50 level pressure of milbemycins, a brown oily mixture (BOM), applied to the egg. Concentration of BOM 4-fold as high as the usual one, however, was sufficient to control the MR-strain living on young kidney bean plants. As a result of the mating test, milbemycin-resistance appears to be controlled by an incompletely dominant gene which exists on the autosome. Under a mixed-rearing test starting from a 1:1 mixture of resistant and susceptible mite populations, resistant population rate was found to increase after one month, but it remained at a stable level during the subsequent nine months. β-Naphthylacetate esterase zymogram pattern of the MR-strain obtained by electrophoresis somehow differed from that of the parent strain. After incubation at 25°C for 24 hours, a preparation of BOM mixed with the homogenate of MR-strain adult mite was less effective than that of the Sapporo S-strain in killing susceptible mites when topically applied to them.

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