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- 仲川 洋治
- 新潟大学医学部細菌学教室
書誌事項
- タイトル別名
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- Studies on Enzymatic Acetylation of Chloramphenicol by Chloramphenicol-Resistant Bacteria
- クロラムフェニコール タイセイキン ニヨル クロラムフェニコール ノ アセチル
- GC-MASSによる解析
この論文をさがす
抄録
Two types of diacetylated chloramphenicol 1, 3-O-O-di [1-12C] acetyl chloramphenicol and 1, 3-O-O-di [1-14C] acetyl chloramphenicol, were subjected to GC-mass analysis. The former showed the presence of prominent mass peaks at m/e 195, 212, 214 and 216, and the latter at m/e 197, 214, 216 and 218. Thus, the fragment ions of the compound diacetylated with. [1-14C] acetyl CoA were detected at a higher mass field by m/e 2 than those of the one diacetylated with [1-12C] acetyl CoA. The C1-C2 bond of diacetylated chloramphenicol was subjected to cleavage. The mass peaks at m/e 195 and 197 and those at m/e 212, 214, 216 and 218 were produced by the fragment containing C-1 and that containing C-3, respectively. The technique was applied to identify the acetoxy group attached to C-1 or C-3 of diacetylated chloramphenicol.<br>To clarify the exact position acetylated by chloramphenicol acetyltransferase in chloramphenicol, the [1-14C] acetyl group wes introduced into 3-O-[1-12C] acetyl chloramphenicol, by using [1-14C] acetyl CoA. The mass peaks of diacetylated chloramphenicol appeared at m/e 195, 212, 214, 216 and 218. Especially, the relative intensity at m/e 214 increased. No peaks occurred, however, at m/e 197. The result described above indicated that the [1-12C] acetyl group was attached to C1-OH and the [1-14O] or the [1-12C] acetyl group to C3-OH in chloramphenicol.<br>From these result and those of kinetic studies, it was considered that both mono- and diacetylation or chloramphenicol by chloramphenicol acetyltransferase might take place in the hydroxy group attached to C-3 of chloramphenicol.<br>The presence of the [1-14C] acetyl group in monoacetylated chloramphenicol suggested a mutual exchange between the [1-12C] acetyl and the [1-14C] acetyl groups.
収録刊行物
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- 日本細菌学雑誌
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日本細菌学雑誌 36 (6), 747-755, 1981
日本細菌学会
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詳細情報 詳細情報について
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- CRID
- 1390001206449419264
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- NII論文ID
- 130001384702
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- NII書誌ID
- AN00189800
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- ISSN
- 18824110
- 00214930
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- NDL書誌ID
- 2476748
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- PubMed
- 6808191
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可