イネ黄萎病ウイルス保毒ツマグロヨコバイの脂肪組織

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  • Adipose Tissue of the Green Rice Leafhopper, <i>Nephotettix cincticeps</i> UHLER, infected with the Virus of the Yellow Dwarf Disease of the Rice Plant
  • イネ オウイビョウ ウィルスホドク ツマグロヨコバイ ノ シボウ ソシキ

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Studies were made in an attempt to find whether there are cytohistological changes in the leafhoppers when they are infected with the virus of the yellow dwarf disease of the rice plant. The results obtained may be summarized as follows:<br>The adipose cells of the affected insect assume a characteristic symptom that distinguishes them from those of healthy individuals. After about 10-15 days of infection the nuclei of the fatty cells become larger in size and somewhat irregular in form. After about 20 days the enlarged nuclei shrink and increase irregularity in form with the simultaneous occurrence of vacuoles of varying sizes in the cytoplasm. Finally after about 25 days whence the virulence of the virus begins to come into play for the plant, the shrinkage of the nuclei as well as of the cells attains the maximum, and the vacuoles increase so enormously in number that the whole cells appear completely reticulation.<br>Cytochemical changes also occur in the same tissue of the viruliferous animales. The FEULGEN reaction as well as the methyl green staining tends to become stronger in intensity at the beginning of the infection, showing presumably the increase of the content of DNA, and the cytoplasm stains heavily with pyronine, suggesting that the increase of RNA content takes place. Then both reaction and staining become less intense, and finally at the highest vacuolar stage of the cytoplasm, they show only the weakest intense.<br>The above mentioned results were obtained withthe exception of a few individuals. Ten adult insects were examined to one batch. The exceptional figure was observed in only 10-20 per cent of the insects.

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