Purification of Staphylococcal Enterotoxin E and Antigenic Similarity between Enterotoxin A and E

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  • SHINAGAWA Kunihiro
    Osaka Prefectural Institute of Public Health College of Agriculture, University of Osaka Prefecture
  • KUNITA Nobuharu
    Osaka Prefectural Institute of Public Health College of Agriculture, University of Osaka Prefecture
  • SAKAGUCHI Genji
    College of Agriculture, University of Osaka Prefecture

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Other Title
  • ぶどう球菌エンテロトキシンＥの精製およびエンテロトキシンＡとＥとの免疫学的交差反応
  • ブドウキュウキン エンテロトキシン E ノ セイセイ オヨビ エンテロトキシン

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Abstract

Staphylococcal food poisoning is caused by ingestion of enterotoxin preformed in foodstuffs. Diagnosis of staphylococcal food poisoning may, therefore, depend upon the detection of enterotoxin in the incriminated foodstuff or in cultures of staphylococci isolated from it or both. For a highly sensitive immunological detection of enterotoxin, specific anti-enterotoxin sera are necessary. Staphylococcal enterotoxin has been classified into five types, A through E, on the basis of antigenicity.<br>Purification of enterotoxin E was first reported by Bergdoll et al. (1971), who stated that enterotoxin E was neutralized with anti-enterotoxin A serum and formed a precipitin line against this serum. Terayama et al. (1974) also purified enterotoxin E and reported that enterotoxin E did not react to anti-enterotoxin A serum.<br>The authors also succeeded in purifying enterotoxin E by essentially the same method as those utilized to purify enterotoxin A, B, and C. Essentially pure enterotoxin E was obtained by three consecutive steps, chromatography on CM-1 and DEAE-Sephadex and twice-repeated gel filtration on Sephadex G-100.<br>Enterotoxin E at a concentration of 120μg/ml formed a distinct precipitin line against anti-enterotoxin A serum. This precipitin line fused with that of enterotoxin E appearing against anti-enterotoxin E serum. At a low concentration of 20μg/ml of the toxin, the precipitin line against anti-enterotoxin A serum and that against anti-enterotoxin E serum coalesced with each other. In contrast, enterotoxin A at 120μg/ml reacted to anti-enterotoxin E serum, against which a precipitin line was formed and fused with that against anti-enterotoxin A serum. When 20μg/ml of this toxin was used, no precipitin line was formed against anti-enterotoxin E serum.

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