Use of the LAMP Method to Discriminate Strain C of <i>Scirtothrips dorsalis</i> (Thysanoptera: Thripidae)

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  • LAMP法によるチャノキイロアザミウマ（アザミウマ目：アザミウマ科）C系統の識別
  • LAMPホウ ニ ヨル チャノ キイロ アザミウマ(アザミウマモク:アザミウマカ)C ケイトウ ノ シキベツ

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Abstract

Strain C of Scirtothrips dorsalis Hood is a novel strain that recently invaded Japan from overseas. To allow simple and rapid identification of this strain, we developed a molecular diagnostic method that uses a loop-mediated isothermal amplification (LAMP) assay. Four primers for the LAMP assay were designed from the nucleotide sequence of the mitochondrial cytochrome c oxidase subunit I (COI) gene of strain C. LAMP assay using these primers was consistently successful in distinguishing strain C from strain YT.

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