Isolation and Toxicity of Lipopolysaccharide of <i>Vibrio parahaemolyticus</i> K minus Strain
-
- SAKAGUCHI Osamu
- Department of Hygienic Chemistry, Tohoku College of Pharmacy
-
- SUZUKI Masuko
- Department of Hygienic Chemistry, Tohoku College of Pharmacy
-
- TAMURA Junichi
- Department of Hygienic Chemistry, Tohoku College of Pharmacy
-
- YOKOTA Katsushi
- Department of Hygienic Chemistry, Tohoku College of Pharmacy
Bibliographic Information
- Other Title
-
- <i>Vibrio parahaemolyticus</i> K<sup>-</sup>株O抗原脂質多糖体の分離と毒性について
- Vibrio parahaemolyticus K-カブ O コウゲン シシツ タトウタイ ノ ブンリ ト ドクセイ ニ ツイテ
Search this article
Description
Lipopolysaccharide (LPS) was extracted from the K minus strain of Vibrio parahaemolyticus with 45% phenol containing 3% NaCl. The resulting crude extract (Fr. I) was further fractionated by centrifugation at 100, 000×g. The precipitated fraction (Fr. II) showed high precipitin activity against O antiserum. Lethality for mice was observed in the Fr. II, but not in the supernatant fraction. The LPS (Fr. II) was hydrolyzed with 1% AcOH at 100° for 1 or 2 hours. The non-dialyzable fraction of the hydrolyzed LPS obtained in a cellophan bag indicated much lower precipitin activity than that of intact LPS.<br>These observations appeared to be the result of liberation of an acid-labile, immunologically active portion from the LPS.<br>Mice injected with the Fr. II died, showing typical endotoxic symptoms and diarrhea.
Journal
-
- Nippon Saikingaku Zasshi
-
Nippon Saikingaku Zasshi 23 (9-10), 715-720, 1968
JAPANESE SOCIETY FOR BACTERIOLOGY
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206454523264
-
- NII Article ID
- 130001383009
-
- NII Book ID
- AN00189800
-
- ISSN
- 18824110
- 00214930
-
- NDL BIB ID
- 8381302
-
- PubMed
- 5751407
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed