Isolation of lactobacillus-phage and its biological characters

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  • 乳酸桿菌ファージの分離ならびに宿主域
  • ニュウサンカンキン ファージ ノ ブンリ ナラビニ ヤドヌシイキ

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Abstract

On the materials gathered from sewages, salivas, dental plaques, and feces of sucklings which may contain lactobucillus, isolation of phage was done. A phage “N-1” was isolated from a material of the sewage.<BR>N-1 phage should have biological characters as followed when examined with L. casei, strain L-10 in L-broth: adsorption rate 73 % at 37°C for 10 minutes, latent period 150 minutes, average but size 46 per cell.<BR>Plaque formation of N-1 phage disturbed by addition of citrate-ion to L-A media as formed less than 1/5 of plaque number of the control. Addition of Ca-ion is essential to have better efficiency of plating.<BR>Survival of N-1 phage in L-broth should decreased to less than 0.1 per cent at 60°C for 5 minutes. Of 40 lactobacillus strains, sensitivity were examined against N-1 phage and J-1 phage which was donated from the Yakuruto Institute. A few strains were detected as sensitive ones against either one of N-1 or J-1 phages respectively.

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