Comparative study on selective media for isolation and quantitative estimation of microorganisms in intestinal flora

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  • 消化管内微生物の分離定量培地に関する研究
  • ショウカ カンナイ ビセイブツ ノ ブンリ テイリョウバイチ ニ カンスル ケンキュウ

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It was revealed that GYB (glucose yeast broth) agar devoid of calcium carbonate was best fitted for counting the total number of viable bacteria in feces, regardless of their kinds. Widely used TGC (thioglycollate) broth was found far inferior to GYB-agar as regards fo: accuracy and sensitivity.<BR>Quantitative estimation and isolation of lactobacilli from human feces were performed most excellently when LBS (Lactobacillus selective medium) 1 agar was used under anaerobic condition. IBS 1 agar is a modified medium of Rogosa's medium (1951), developed by the authors in order for better isolation of lactobacilli. It contains, besides LBS medium, liver extract, lactose and cysteine.<BR>As for estimation of lactic acid bacteria (lactobacilli and streptococci), our tomato juice agar containing 0.1% furan-acrylic acid gave most excellent results.<BR>Concomitant studies on various selective media currently used for other microbes confirmed that for enteric bacteria, MacConkey, Desoxycholate agar, SF (Streptococcus faecalis) medium as well as Staphylococcus medium No.110; for yaests and Candida, potato glucose agar or CGS (Sabouraud's medium containing guanoflacine) agar; were excellent selective media, respectively.

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