腸内細菌の薬剤耐性

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タイトル別名
  • Drug-Resistance of Enteric Bacteria
  • チョウ ナイ サイキン ノ ヤクザイ タイセイ
  • Transduction of transmissible drug-resistance (R) factor with phage epsilon
  • ε<SUB>15</SUB>およびε<SUB>34</SUB> phageによるR因子の導入について

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説明

Transmissible drug-resistance (R) factor was transduced in the system of SalmonellaE group with phage epsilon, and its transduction rate was 10-8.<BR>The transduction rate of R factor with epsilon phage that had existed as prophage in R+host cell and was induced by UV irradiation increased about 102 times than that of ordinary transduction.<BR>The R factor carrying TC resistance marker was consistently segregated by transduction with epsilon phage.<BR>The R+transductant was not lysogeniged by transducing epsilon phage, indicating non-lysogenic conversion and non-immunity toward the transducing epsilon phage.<BR>The R factor of transductant was non-transmissible by conjugation and not eliminated by acriflavine treatment. But the R factor of transductant was transduced again by epsilon phage that had existed as prophage in R+transductant or that was newly infected to R+transductant.<BR>The R factor of R+transductant of S. chittagongwas transmissible by conjugation at 106times lower frequency than that of R factor that was transferred by conjugation, and was eliminated by acriflavine treatment in low frequency.<BR>In transduction of R factor R+ transductant with the same epsilon phage that had transduced high frequency transductant (HFT) was obtained and its transduction rate was 10-2.<BR>The transmission of R factor to R+transductant was higher than that to R-or R+cell to which R factor was transmitted by conjugation.

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