Antibiotic Treatment of Experimental Tularemia in Mice with Special Reference to Strong Immunity Established after Treatment

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  • 実験野ウサギ病の抗生物質療法と治療後の免疫成立について
  • ジッケン ヤトビョウ ノ コウセイ ブッシツ リョウホウ ト チリョウゴ ノ

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Abstract

A study was made on the antibiotic treatment of experimental tularemia in mice. The antibiotics tested were streptomycin, oxytetracycline, tetracycline, kanamycin, chloramphenicol, leucomycin, and erythromycin. Mice (ddK) were inoculated subcutaneously with 104 MLD of the virulent strain (Ebina) of Francisella tularensis. The antibiotic treatment started 48 hours after inoculation. It was subcutaneous injection with a given dose of drug every 6 hours for 10 days. It presented the following results: (1) The antibiotics tested were all effective when administered in sufficient doses. (2) The therapeutic efficacy of the antibiotics appeared to have little relation with their permeability into cells, but to be almost proportionate to their bacteristatic activity in vitro. This result would be in conflict with the opinion that F. tularensis is an intracellular parasite. (3) The drug sensitivity and virulence of the organisms isolated from survived mice were equivalent to those of the original Ebina strain. (4) After the antibiotic treatment of tularemia, a strong immunity was established in survived mice. These mice could defend themselves against as large a challenge dose as 109 MLD of the virulent strain. (5) Immunization with formalin-killed organisms of the virulent strain and with living organisms of the avirulent strain gave rise to such a strong protective immunity in mice as comparable to that established by immunization with living virulent organisms through antibiotic therapy.

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