鶏射出精子の性状および受精能力におよぼす雄鶏血清の影響

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  • Effect of Cock Serum on Motility, Deformity and Fertility of Ejaculated Cock Spermatozoa
  • ケイ シャシュツ セイシ ノ セイジョウ オヨビ ジュセイ ノウリョク ニ オヨボス オンドリ ケッセイ ノ エイキョウ

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Present studies were carried out to clarify the effects of cock serum on the motility, deformity and fertility of cock spermatozoa.<br>Semen was collected from 10-15 roosters by the method of abdominal massage. The semen was centrifuged for 5 minutes at a speed of 3, 000r.p.m, and the spermatozoa were washed twice with phosphate buffer (δ=0.615, pH=7.17) and resuspended in cock serum diluted at different rates with the phosphate buffer. Observation and insemination were performed just after treatment or after storage at 0°C for one to six days.<br>The results obtained are as follows:<br>(1) When the washed spermatozoa were resuspended in phosphate buffer, motility of spermatozoa was extremely suppressed. However, fertility was not affected.<br>(2) Motility of washed spermatozoa, which was suppressed by phosphate buffer, was wholly regained with addition of cock serum.<br>(3) Cock serum diluted 6- or 8-fold with phosphate buffer was highly effective for the storage of washed spermatozoa, compared to undiluted cock serum and phosphate buffer.<br>(4) Fertility of washed spermatozoa stored for 1 day at 0°C was higher in cock serum diluted 6- or 8-fold (36-81%) than in phosphate buffer (0-24%) and undiluted cock serum (0%).<br>These results show that the addition of cock serum in a low concentration to phosphate buffer is useful for the storage of cock spermatozoa in vitro.

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