ヒメウズラ,野生系ウズラとニワトリ白色ブロイラー間のシトクロームb領域におけるPCR-RFLP解析

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  • Comparison of Cytochrome b Region Among Chinese Painted Quail, Wild-Strain Quail, and White Broiler Chicken Based on PCR-RFLP Analysis.

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PCR-RFLP analysis was used to examine 1, 075 bp cytochrome b (Cyt b) region of mtDNA in each of 20 birds of Chinese painted quail (Excalfactoria chinensis), wild-strain Japanese quail (Coturnix japonica), and white broiler chicken (Gallus gallus). The total Cyt b amplicon was digested with 10 restriction endonucleases, and its electrophoretic pattern was investigated. Ten kinds of restriction enzyme digestions were identical among 20 individuals in each of the three species. No variation was observed in the 10 kinds of enzyme digestions among different individuals within the three species in-vestigated. The different specific electrophoretic patterns, haplotypes, and total restric-tion fragments were observed in each of the three species belonging to the same family Phasianidae. The result representatives the evolutionary genetic characteristics of RFLP of cytochrome b region in the three different species, and indicated the genetic diversity among the three genuses of the family Phasianidae, and genetic identity within each of the species. The result could be used for the identification of different species within family Phasianidae and as a reference of physical map for cytochrome b gene.

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