低カルシウム飼料給餌による卵殻質の低下と小腸及び卵殻腺におけるカルシウム結合タンパク質 (CaBP-D<sub>28K</sub>)遺伝子発現に与える影響

  • 家田 照子
    名古屋大学大学院生命農学研究科
  • 齋藤 昇
    名古屋大学大学院生命農学研究科
  • 島田 清司
    Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University

書誌事項

タイトル別名
  • Effect of Low Calcium Diet on Messenger Ribonucleic Acid Levels of Calbindin-D28K of Intestine and Shell Gland in Laying Hens in Relation to Egg Shell Quality.
  • Effect of Low Calcium Diet on Messenger Ribonucleic Acid Levels of Calbindin-D28K of Intestine and Shell Gland on Laying Hens in Relation to Egg Shell Quality

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We investigated the effects of short-term feeding low calcium diet on egg shell quality (specific gravity, relative weight of egg shell, egg shell thickness) and mRNA levels of calbindin-D28K (CaBP-D28K) in the shell gland and intestine. After feeding low calcium diet (0.5%), hens laid fragile eggs with low shell quality. Plasma calcium concentration also decreased significantly after feeding of low calcium diet. When the hens received a moderate calcium diet (3.5%) after low calcium diet for 5 days, shell quality was improved and plasma calcium concentrations increased. Levels of CaBP-D28K mRNA in the shell gland did not change after feeding low calcium diet for 5 days. Levels of CaBP-D28K mRNA in the intestine of the low calcium diet group significantly increased 2 fold when compared to those of the control group. We infer that low calcium concentration in plasma may stimulate parathyroid and 1, 25(OH)2D3 production in the kidney and induce CaBP-D28K mRNA expression in the intestine. It is supposed that a low calcium diet influences egg shell quality, plasma calcium concentration and intesti-nal CaBP-D28K gene expression by calcium homeostasis. In the shell gland, however, the levels of CaBP-D28K mRNA may not be influenced by low plasma calcium.

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