Biosensor Device with SPR Imaging Technique: The Design of High Density DNA Bio-tip

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  • TAMADA Kaoru
    Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology Advanced Industrial Science and Technology (AIST), Photonics Research Institute
  • ITO Masateru
    Advanced Industrial Science and Technology (AIST), Photonics Research Institute
  • NAKAMURA Fumio
    Advanced Industrial Science and Technology (AIST), Photonics Research Institute PRESTO, Japan Science and Technology (JST)
  • BABA Akira
    Departments of Chemistry, University of Houston

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Other Title
  • 「化学センサの現状と展望」ＳＰＲイメージング法のバイオセンサー応用：高密度集積型ＤＮＡマイクロアレイの開発
  • SPRイメージング法のバイオセンサー応用:高密度集積型DNAマイクロアレイの開発
  • SPR イメージングホウ ノ バイオセンサー オウヨウ コウミツド シュウセキガタ DNA マイクロアレイ ノ カイハツ

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Abstract

We have developed a new methodology to fabricate high resolution DNA microarray (2,500-104 dots/cm2) in combination with micro contact printing (μ-CP) and SPR imaging. Novel COOH-terminated PEG-disulfides with p-carborane were synthesized to fabricate the first layer on gold substrates to achieve surface coupling reaction with amino-terminated DNA in high yield. The hybridization of the target-DNA modified gold nanoparticles on probe-DNA patterned surfaces was successfully demonstrated by the SPR imaging, where nonspecific adsorption was not observed to the array background. The gold nanoparticle arrays provide quite high contrast even at low surface coverage (∼10%) by the enhancement effect of optical signals based on nano-scale phenomena in the near field. This enhancement effect can be well demonstrated by the simulation based on Maxwell-Garnett (MG) theory and Fresnel's equation.

Journal

  • Hyomen Kagaku

    Hyomen Kagaku 27 (1), 21-26, 2006

    The Surface Science Society of Japan

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