Antibody Detection against Red Sea Bream Iridovirus (RSIV) in Yellowtail Seriola quinqueradiata Using ELISA

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  • ELISA法による抗RSIVブリ抗体の検出

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Abstract

FBS components in viral culture fluid interfere with the detection of specific antibody in immunized fish. In the present study, we developed a method to eliminate the interference of FBS using a model of red sea bream iridovirus (RSIV) vaccine. In an experiment using yellowtail antiserum against BSA, which is a major component of FBS in cell culture media, antibodies against BSA were completely absorbed via incubation of antiserum in 50% FBS at 25°C for 1 h. In antibody detection, ELISA with the sera of yellowtail surviving RSIV-challenge, antibodies against RSIV were detected, but those against FBS disappeared via the pre-treatment of the sera. Therefore, antibodies against RSIV are effectively detected using ELISA with the pre-treatment of fish sera.

Journal

  • Fish Pathology

    Fish Pathology 45 (2), 73-76, 2010

    The Japanese Society of Fish Pathology

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