飼料用マイワシ筋肉からのハマチ病原性Ｓｔｒｅｐｔｏｃｏｃｃｕｓ　ｓｐ．の分離

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タイトル別名
  • Occurrence of Streptococcus sp., a Pathogen of Cultured Yellowtail, in Muscle of Sardine for Diets
  • シリョウヨウ マイワシ キンニク カラ ノ ハマチ ビョウゲンセイ Strep

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説明

The dorsal muscle of sardine(Sardinops melanosticta), frozen as fish diets, was subjected to detection of Streptococcus sp., a pathogen of cultured yellowtail (Seriola quinoueradiata). After the external surface of sardine was disinfected with 70%ethanol, 2-3 g of samples were cut aseptically from the dorsal muscle. The muscle samples were dipped in 70%ethanol and then flamed for disinfecting its surface. Immediately after that, each of the samples were inoculated into nutrient broth, and then incubated for 1-5 days at 25°C. Subsequently, a loopful of the culture was streaked on nutrient agar, and then incubated for 1-2 days at 25°C. Cultures on the plates consisted of various kinds of colonies. On most plates however, pure culture of the objective organisms was observed.<BR>As a result, Streptococcus sp. was isolated from all of the 10 sardines sampled. Such remarkable frequency in its detection may be attributed to the enrichment culture using ordinary broth and prolonged incubation.<BR>The role of the organism in streptococcal infection-of yellowtail and the cause of its isolation from the sardine muscles as feeds remained to be further clarified in the future.

収録刊行物

  • 魚病研究

    魚病研究 17 (3), 195-198, 1982

    日本魚病学会

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