Localization Analysis of Fluorescent-labeled Aeromonas veronii in the Intestinal Tract of Carp using an in vivo Imaging System

  • Namba Aki
    Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
  • Mano Nobuhiro
    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
  • Hirose Hitomi
    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
  • Nakanishi Teruyuki
    Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

書誌事項

タイトル別名
  • Localization Analysis of Fluorescent-labeled <i>Aeromonas veronii</i> in the Intestinal Tract of Carp using an <i>in vivo</i> Imaging System

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抄録

Aeromonas veronii is a Gram-negative opportunistic pathogen in fish. We have previously investigated the intestinal microbiota of carp Cyprinus carpio and revealed that most bacterial isolates with high mucus-binding capability were A. veronii. With regard to infection, mucosal adhesion of bacteria is regarded as a prerequisite for colonization and invasion. In the present study, we selected three A. veronii strains with different mucus-binding capability and pathogenicity, and compared the colonization capability and localization of these strains in the intestinal tract of carp, using an in vivo imaging system. We showed that only a pathogenic strain was mainly visible around the intestinal bulb and rectum of the intestinal tract at 48 h after administration. Frozen sections with fluorescence signals revealed that the administered bacteria were localized on the mucosal surfaces and in the lamina propria of the intestinal bulb, whereas they were mainly detected in the remaining intestinal contents in the rectum. These results suggest that the intestinal bulb is the primary region for A. veronii to invade and proliferate in healthy carp.

収録刊行物

  • 魚病研究

    魚病研究 47 (1), 7-11, 2012

    日本魚病学会

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