Studies on the method for evaluating virucidal activities of germicides to IPNV and IHNV.
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- INOUYE Kiyoshi
- Iki Branch Bureau, Nagasaki Prefectural Office
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- IKEYA Fumio
- Okutama Branch, Tokyo Metropolitan Fishery Experimet Station
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- YAMAZAKI Takayoshi
- Nagano Prefectural Fishery Experiment Station
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- HARA Takeshi
- National Research Institute of Aquaculture
Bibliographic Information
- Other Title
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- サケ科魚類の伝染性病原体の消毒法に関する研究 1 IPNウイルスおよびIHNウイルスに対する消毒剤の効力評価法に関する検討
- IPN ウイルス オヨビ IPN ウイルス ニ タイスル ショウドクザイ ノ
- サケ科魚類の伝染性病の消毒法に関する研究1
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Abstract
Optimum methods for evaluating virucidal activities of germicides to infectious pancreatic necrosis virus (IPNV) and infectious hematopoietic necrosis virus (IHNV) were studied.<BR>1. Among the fish cell lines examined CHSE-214 and EPC cells were best suited for examining virucidal activities.<BR>2. Cell-associated virus suspension (CAV) was effectively obtained by freezing and thawing cell-sheet culture just before the appearance of cytopathological effects (CPE).<BR>3. CAV of IPNV did not change in infectivity titer during the stocking period of 8 weeks at either -20°C or -80°C. However, infectivity titer of IHNV in CAV significantly decreased infectivity titer at both -20°C and -80°C.<BR>4. Virucidal activities of IPNV and IHNV did not change during 6 h incubation at temperatures from 10°C to 37°C and from 10°C to 15°C, respectively. Hence, 15°C appears to be a reasonable temperature for routine use in this assay system.<BR>5. Toxicities to IPNV and IHNV of germicides other than halogenic compounds were successfully eliminated by 102-105 dilutions of PBS (-).
Journal
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- Fish Pathology
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Fish Pathology 25 (2), 69-79, 1990
The Japanese Society of Fish Pathology
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Details 詳細情報について
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- CRID
- 1390001206460009856
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- NII Article ID
- 130000857876
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- NII Book ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL BIB ID
- 3671304
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed