ニホンウナギ(Anguilla juponica)卵巣由来細胞株について

  • 陳 秀男
    中華民國台湾台北市國立台湾大学動物学系
  • 郭 光雄
    中華民國台湾台北市國立台湾大学動物学系

書誌事項

タイトル別名
  • A cell line derived from Japanese eel (Anguilla japonica) ovary.
  • ニホンウナギ(Anguilla japonica)卵巣由来細胞株について〔英文〕
  • ニホンウナギ Anguilla japonica ランソウ ユライ サイボウシ

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説明

A continuous cell line, designated EO-2, was established from the ovary of Japanese eel, Anguilla japonica. The cells have been passed for 110 times during 19 months in Leibovitz's L-15 plus 10% foetal calf serum under a temperature of 32±1°C. The cell line consists of fibroblastic cells with large or small nuclei. Multinucleated cells were present in most of the cultures. The number of chromosome counted from 100 EO-2 metaphase cells was ranging from 22 to 74 with a modal number of 38. A low plating efficiency of approximately 8% was recorded for EO-2 cell at 32±1°C. The temperature ranged from 20 to 37°C could support the growth of EO-2 cell line.<BR>EO-2 cells were also demonstrated to be susceptible to Eel Virus Europe (EVE), Eel Virus Europe X (EVEX) and Eel Virus America (EVA). In comparison, EO-2 cells are more sensitive to these three viruses than RTG-2 or FHM cells. The cell line was found to be free of bacterial, fungal and mycoplasma contamination.

収録刊行物

  • 魚病研究

    魚病研究 16 (3), 129-137, 1981

    日本魚病学会

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