Molecular Identification of Marine Crustacean-pathogenic Peronosporomycetes Using DNA Sequences of ITS1 and their Pathogenicity for Nauplii of Brine Shrimps

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  • Muraosa Yasunori
    Division of Clinical Research, Medical Mycology Research Center, Chiba University Laboratory of Fish Diseases, Nippon Veterinary and Life Science University
  • Sano Ayako
    Department of Animal Sciences, Faculty of Agriculture, University of the Ryukyus
  • Hatai Kishio
    Laboratory of Fish Diseases, Nippon Veterinary and Life Science University

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Other Title
  • 海生甲殻類から分離した病原卵菌類のITS1領域の塩基配列による同定とアルテミア孵化幼生に対する病原性

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Description

We isolated 27 strains of marine crustacean-pathogenic Peronosporomycetes from various infected marine crustaceans collected in Japan, Thailand, Vietnam and the Philippines, and identified them morphologically. The internal transcribed spacer (ITS) l region of the nuclear rRNA gene of the 27 strains and 12 reference strains of six peronosporomycete species isolated from marine crustaceans were sequenced. The phylogenic relationship inferred from the sequences corresponded well to the morphological identification, indicating that sequencing of the ITS1 region is a useful tool for identification of marine crustacean-pathogenic Peronosporomycetes. Nauplii of brine shrimp Artemia salina were experimentally challenged by bathing in zoospore suspensions (1 × 104 zoospores/mL, 25°C) with 18 strains belonging to nine species in four genera. The 48-h mortalities varied from 1.1% to 99.4% among the species, though all the strains were isolated from diseased animals.

Journal

  • Fish Pathology

    Fish Pathology 47 (2), 41-48, 2012

    The Japanese Society of Fish Pathology

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