PCR-RFLP Analysis for Identification of Aeromonas Isolates Collected from Diseased Fish and Aquatic Animals

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  • Rahman Mahbubur
    Laboratory of Microbiology, Graduate School of Fisheries Sciences, Hokkaido University Department of Biotechnology, Shah Jalal University of Science and Technology
  • Somsiri Temdoung
    Aquatic Animal Health Research Institute, Kasetsart University campus
  • Tanaka Reiji
    Laboratory of Microbiology, Graduate School of Fisheries Sciences, Hokkaido University
  • Sawabe Tomoo
    Laboratory of Microbiology, Graduate School of Fisheries Sciences, Hokkaido University
  • Tajima Kenichi
    Laboratory of Microbiology, Graduate School of Fisheries Sciences, Hokkaido University

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Other Title
  • エロモナス属菌のPCR-RFLP解析による同定

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Abstract

The study was conducted to develop a PCR and an improved PCR-RFLP analysis method for rapid species-identification of Aeromonas genospecies. The forward and reverse primers for PCR were designed from the complementary sequences of the 16S rDNA of all 15-recognized Aeromonas genospecies to amplify a 1206-bp PCR product. The PCR amplified the expected product from the DNA template of type or reference strains of all recognized Aeromonas genospecies as well as 106 Aeromonas strains, while no PCR product was obtained from any of the non-Aeromonas strains tested. The PCR-RFLP analysis using the restriction enzymes (Alul, Mbol, Pvull, Pstl and Narl) provided identification of almost all Aeromonas species. However, the Aeromonas sp. T8 group and A. caviae exhibited a similar RFLP pattern. Some selected biochemical tests were found to be helpful for differentiation of the two species.

Journal

  • Fish Pathology

    Fish Pathology 40 (4), 151-159, 2005

    The Japanese Society of Fish Pathology

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