ウナギの好中球におけるグリコーゲン分解酵素とグリコーゲン合成酵素の活性

DOI Web Site

書誌事項

タイトル別名
  • Activities of glycogen phosphorylase and glycogen synthetase in eel neutrophils.
  • ウナギ ノ コウチュウキュウ ニ オケル グリコーゲン ブンカイ コウソ ト

この論文をさがす

説明

The existence of glycogen phosphorylase and glycogen synthetase was histochemically demonstrated in the neutrophils of eel (Anguilla japonica) under inflammatory condition by the reaction of polysaccharide derived from glucose-1-phosphate or uridine diphosphate glucose with iodine and PAS, respectively. The enzyme activities were also determined in the peripheral and pronephros neutrophils obtained from the fish after an intraperitoneal injection of formalinkilled Edwardsiella tarda or physiological saline. The activity of the glycogen phosphorylase was measured by the amount of the released inorganic phosphate from glucose-1-phosphate, and that of the glycogen synthetase by the amount of the released uridine diphosphate from uridine diphosphate glucose.<BR>In the pronephros neutrophils from the fish injected with formalin-killed E. tarda, the reaction of glycogen phosphorylase became prominent between 24 and 48 hours after injection, while that of glycogen synthetase increased conspicuously from 3 hours. But there were no significant differences in the reactions of two enzymes between the control fish injected with physiological saline and normal fish.<BR>The activities of glycogen phosphorylase and glycogen synthetase in the peripheral neutrophils did not differ between the bacteria-injected and control fish, but those, especially D-form of glycogen synthetase, in the pronephros neutrophils were greatly increased in the bacteria-injected fish.<BR>From the results of histochemical reactions and enzyme activities of these two enzymes, it is suggested that the increase in glycogen contents of neutrophils under inflammatory condition resulted from the activation of D-form of glycogen synthetase which had been achieved in the pronephron before being emigrated into the peripheral blood.

収録刊行物

  • 魚病研究

    魚病研究 26 (1), 35-43, 1991

    日本魚病学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ