Activity of Granulocytes and Chemokines in the Leukocyte-encapsulation Response of Japanese Flounder Paralichthys olivaceus
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- Kurata Osamu
- Laboratory of Fish Diseases, Department of Veterinary Science, Nippon Veterinary and Life Science University
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- Kitancharoen Nilubol
- Department of Fisheries, Faculty of Agriculture, Khon Kaen University
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- Fujiwara Atushi
- National Research Institute of Aquaculture, Fisheries Research Agency
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- Nakayasu Chihaya
- National Research Institute of Aquaculture, Fisheries Research Agency
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- Wada Shinpei
- Laboratory of Fish Diseases, Department of Veterinary Science, Nippon Veterinary and Life Science University
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- Hatai Kishio
- Laboratory of Fish Diseases, Department of Veterinary Science, Nippon Veterinary and Life Science University
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説明
The role of leukocytes from the kidney of Japanese flounder Paralichthys olivaceus in the encapsulation response was investigated using an in vitro model. The aggregation response of leukocytes to the target fish pathogen Ichthyophonus hoferi was observed over 12 h. The cellular aggregates mainly comprised granulocytes, which were characterized by intracellular peroxidase expression. Moreover, studies of isolated granulocytes demonstrated their adhesion to I. hoferi and formation of an initial layer in the encapsulation response. We observed that mRNA expression levels of three CC-chemokines (CCL3, CCL4 and CC-CLM) and one CXC-chemokine (IL-8) increased during the encapsulation process. Among these chemokines, CC-CLM and IL-8 were actively produced by granulocytes upon stimulation by I. hoferi. Flounder granulocytes therefore appeared to play an important role in the process by releasing specific chemokines upon pathogen recognition, thereby inducing subsequent cellular recruitment leading to encapsulation.
収録刊行物
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- 魚病研究
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魚病研究 45 (3), 121-129, 2010
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390001206460921344
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- NII論文ID
- 10026600679
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL書誌ID
- 10833946
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可