-
- Sung Hung-Hung
- Department of Microbiology, Soochow University
-
- Kuo Pei-An
- Department of Microbiology, Soochow University
-
- Kao Wen-Yi
- Department of Microbiology, Soochow University
書誌事項
- タイトル別名
-
- Effect of Lipopolysaccharide on In Vitro Phagocytosis by Hemocytes from Giant Freshwater Prawn (Macrobrachium rosenbergii).
この論文をさがす
抄録
When separate batches of Macrobrachium rosenbergii hemocytes were cultured in different media at 25°C for 40 min, the percentage of viable hemocytes cultured in modified M-199 medium (M-199;80%) was significantly higher than the percentage cultured in modified crayfish saline (M-CFS;65%), although wide individual variation was noted. The percentage of phagocytic cells increased significantly with lipopolysaccharide (LPS) concentrations up to 16μg/mL, but no further significant increases were found at concentrations from 16 to 100μg/mL. Microscopy of phagocytosis in hemocytes cultured in M-199 and induced with LPS at a final concentration of 20μg/mL showed that target zymosan particles (1-4μm) were engulfed by granulocytes (GC) and semigranulocytes (SGC), but were only attached to and not engulfed by hyaline cells (HC). However, smaller sized fluorescent-labeled beads (0.5μm) were primarily phagocytosed by HC. This suggests that all three types of hemocytes from M. rosenbergii are able to phagocytose foreign particles. Microscopy showed that LPS significantly altered hemocyte morphology by increasing pseudopodia stretching and hemocyte size and it also increased the number of engulfed particles per phagocyte. The percentage of HC positive for two lysosomal enzymes (acid phosphatase and α-naphthyl acetate esterase) related to phagocytosis was higher than that of positive SGC or GC.
収録刊行物
-
- 魚病研究
-
魚病研究 35 (3), 109-116, 2000
日本魚病学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206461236608
-
- NII論文ID
- 10019222181
-
- NII書誌ID
- AN00063165
-
- ISSN
- 18817335
- 0388788X
-
- NDL書誌ID
- 5477573
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可