Indirect Enzyme-linked Immunosorbent Assay (ELISA) for the Detection of Eel Serum Antibody.
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- Iida Takaji
- Department of Fisheries, Faculty of Agriculture, The University of Tokyo
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- Yonekura Hiroyuki
- Department of Fisheries, Faculty of Agriculture, The University of Tokyo
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- Izumiyama Masafumi
- Department of Fisheries, Faculty of Agriculture, The University of Tokyo
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- Wakabayashi Hisatsugu
- Department of Fisheries, Faculty of Agriculture, The University of Tokyo
Bibliographic Information
- Other Title
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- 間接ELISA法によるウナギ抗体の検出
- 間接ELISA法によるウナギ抗体の検出〔英文〕
- カンセツ ELISAホウ ニ ヨル ウナギ コウタイ ノ ケンシュツ エイブン
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Description
Immunoglobulin (Ig) was purified from the serum of eel (Anguilla japonica) and an indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of eel antibody. Sera were collected from eel which had been injected with formalin-killed Edwardsiella tarda bacterin. A pool of the sera was precipitated with a 40% saturated ammonium sulfate solution, and then immunoglobulin was purified from the precipitates by gel-filtration and ion-exchange chromatography. The purified Ig emulsified in Freund's complete adjuvant was administered to a rabbit to prepare anti-eel Ig serum.<BR>Eel were immersed in the E. tarda bacterin at a density of 1 mg wet weight/ml for 1 h. Change in antibody titers in the vaccinated fish was determined by indirect ELISA. The titers of vaccinated fish were significantly higher than those of unvaccinated control fish.
Journal
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- Fish Pathology
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Fish Pathology 26 (4), 201-205, 1991
The Japanese Society of Fish Pathology
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Details 詳細情報について
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- CRID
- 1390001206461310592
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- NII Article ID
- 130000857455
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- NII Book ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL BIB ID
- 3748920
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed