Comparison of Genome Size and Synthesis of Structural Proteins of Hirame Rhabdovirus, Infectious Hematopoietic Necrosis Virus, and Viral Hemorrhagic Septicemia Virus.

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Other Title
  • HRV,IHNV,VHSVのRNAサイズおよび感染細胞内での構造蛋白の代謝
  • HRV,IHNV,VHSVのRNAサイズおよび感染細胞内での構造蛋白の代謝〔英文〕
  • HRV IHNV VHSV ノ RNA サイズ オヨビ カンセン サイボウナイ

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Genomic RNA was extracted from purified virions of hirame rhabdovirus (HRV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The full-length RNA was analyzed using formaldehyde agarose gel electrophoresis followed by ethidium bromide staining. Compared with an internal RNA size standard, all three viral genomic RNAs appeared to have identical relative mobilities and were estimated to be approximately 10.7 kilobases in length or about 3.7 megadaltons in molecular mass. Structural protein synthesis of HRV, IHNV, and VHSV was studied using cell cultures treated with actinomycin D. At 2 h intervals, proteins were labeled with 35S-methionine, extracted, and analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography. The five structural proteins of each of the three viruses appeared in the following order : nucleoprotein (N), matrix protein 1 (M1), matrix protein 2 (M2), glycoprotein (G), and polymerase (L) reflecting both the approximate relative abundance of each protein within infected cells and the gene order within the viral genome.

Journal

  • Fish Pathology

    Fish Pathology 26 (2), 77-81, 1991

    The Japanese Society of Fish Pathology

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