アサリに寄生するウミグモ幼生の鋭敏な検出法の開発

DOI 日本農学文献記事索引 Web Site 被引用文献2件 参考文献12件
  • Yoshinaga Tomoyoshi
    Department of Aquatic Bioscience, Graduate School of Agricultural Life Sciences, The University of Tokyo
  • Kobayashi Yutaka
    Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center
  • Toba Mitsuharu
    Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center
  • Miyama Yoshifumi
    Tokyo Bay Fisheries Laboratory, Chiba Prefectural Fisheries Research Center

書誌事項

タイトル別名
  • Development of a Sensitive Method for the Detection of Young Larvae of the Parasitic Pycnogonid Nymphonella tapetis in Manila Clam Ruditapes philippinarum

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抄録

We developed a sensitive method for the detection of Nymphonella tapetis (sea spider) larvae in Manila clam Ruditapes philippinarum. The gills were removed from infected clams and lysed in 2N NaOH at 60°C. The lysate was centrifuged and the pellet was stained in 0.2% Uvitex 2B solution and observed under a fluorescent microscope. Using this method, we were easily able to enumerate the larvae. The majority of larvae were found in the gills, which were indicated as the primary site of infection for N. tepetis larvae.

収録刊行物

  • 魚病研究

    魚病研究 46 (1), 38-41, 2011

    日本魚病学会

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